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L-canavanine was added to GA_3 treated barley seeds, and induced amylase activity, soluble protein content, and arginine content were measured. Canavanine, added at the beginning of the incubation period, inhibited amylase activity and protein accumulation. Amylase activity decreased markedly by addition of canavanine at 6 hr after incubation, where soluble protein content was not affected. The addition of canavanine after 12 hr incubation did not show serious inhibitory effect on the amylasa activity and protein accumulation. GA_3 incubation caused decrement in arginine content per ㎎ protein, but it was somewhat recovered by canavanine treatment. The longer the time between GA_3 and canavanine addition was, the less the recovery ratio was. Arginine content in the α-amylase fraction (ammonium sulfate 20∼50% saturation) was lower than in 0∼20% fraction, but higher than in 50∼80% fraction. These results and control experiments, using cordycepin and cycloheximide, support the idea that canavanine might incorporate into protein.
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